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Características hematológicas de seis espécies de quelônios criados em cativeiro no município de Belém-Pa

2026-09-25T11:57:39Z

Título: Características hematológicas de seis espécies de quelônios criados em cativeiro no município de Belém-Pa Autor(es): CANELAS, Vitória Abstract: Hematology is the science dedicated to the study of blood, its origin, and its cellular components, allowing for the evaluation of the physiological, immunological, and metabolic state of the organism, as well as assisting in the diagnosis, prognosis, and monitoring of various diseases. Among hematological tests, the complete blood count (CBC) is the most frequently requested, as it provides an overview of the animal's overall condition. In wild animals, the evaluation of the blood smear is considered the most important step in performing this test, since it is at this stage that abnormalities in red blood cells, morphological changes in white blood cells, and the presence of cellular inclusions and hemoparasites can be visualized. Considering the scarcity of specific studies and the great morphological variability of blood cells in wild species, microscopic analysis of the blood smear becomes fundamental for the correct interpretation of hematological results. Thus, the objective of this research is to contribute to specific studies focused on the hematology of Testudines found in the Amazon region through photodocumentation of blood smears, complete blood count, and blood gas analysis, in order to provide greater ease in the analysis, identification of cells, and interpretation of the examinations of these animals. The research was carried out by obtaining blood samples from 6 species of Testudines: Chelonoidis carbonaria (red-footed tortoise), Chelonoidis denticulata (yellow-footed tortoise), Podocnemis expansa (Amazonian turtle), Rhinoclemmys punctularia (perema turtle), Podocnemis unifilis (yellow-spotted river turtle), and Kinosternon scorpioides (scorpion turtle), obtained from the Museu Paraense Emílio Goeldi. Blood smears were performed on all species, without the use of anticoagulants, stained using the Rapid Panoptic® and adapted May-Grünwald-Giemsa techniques, subsequently analyzed and photomicrographed. Complete blood counts and blood gas analyses were performed on the species Jabuti-Tinga, Jabuti-Piranga, and Amazon River Turtle. Complete blood counts were sent to a partner laboratory, and blood gas analyses were performed locally by venipuncture using the Seamaty Blood Gas & Electrolyte Analyzer - Model VG1. Cell identification demonstrated the efficiency of MayGrunwald-Giemsa staining compared to Panoptic Rapid®, in addition to corroborating and adding relevant information about the morphology of the cellular components of the blood of these animals. The complete blood count and blood gas analysis values found in this study corroborated established literature and added new data about chelonians in the Northern region of Brazil.; Hematology is the science dedicated to the study of blood, its origin, and its cellular components, allowing for the evaluation of the physiological, immunological, and metabolic state of the organism, as well as assisting in the diagnosis, prognosis, and monitoring of various diseases. Among hematological tests, the complete blood count (CBC) is the most frequently requested, as it provides an overview of the animal's overall condition. In wild animals, the evaluation of the blood smear is considered the most important step in performing this test, since it is at this stage that abnormalities in red blood cells, morphological changes in white blood cells, and the presence of cellular inclusions and hemoparasites can be visualized. Considering the scarcity of specific studies and the great morphological variability of blood cells in wild species, microscopic analysis of the blood smear becomes fundamental for the correct interpretation of hematological results. Thus, the objective of this research is to contribute to specific studies focused on the hematology of Testudines found in the Amazon region through photodocumentation of blood smears, complete blood count, and blood gas analysis, in order to provide greater ease in the analysis, identification of cells, and interpretation of the examinations of these animals. The research was carried out by obtaining blood samples from 6 species of Testudines: Chelonoidis carbonaria (red-footed tortoise), Chelonoidis denticulata (yellow-footed tortoise), Podocnemis expansa (Amazonian turtle), Rhinoclemmys punctularia (perema turtle), Podocnemis unifilis (yellow-spotted river turtle), and Kinosternon scorpioides (scorpion turtle), obtained from the Museu Paraense Emílio Goeldi. Blood smears were performed on all species, without the use of anticoagulants, stained using the Rapid Panoptic® and adapted May-Grünwald-Giemsa techniques, subsequently analyzed and photomicrographed. Complete blood counts and blood gas analyses were performed on the species Jabuti-Tinga, Jabuti-Piranga, and Amazon River Turtle. Complete blood counts were sent to a partner laboratory, and blood gas analyses were performed locally by venipuncture using the Seamaty Blood Gas & Electrolyte Analyzer - Model VG1. Cell identification demonstrated the efficiency of MayGrunwald-Giemsa staining compared to Panoptic Rapid®, in addition to corroborating and adding relevant information about the morphology of the cellular components of the blood of these animals. The complete blood count and blood gas analysis values found in this study corroborated established literature and added new data about chelonians in the Northern region of Brazil.

Acesso cirúrgico para osteossíntese diafisária de ossos longos do membro torácico de Tamandua tetradactyla.

2026-03-05T19:58:04Z

Título: Acesso cirúrgico para osteossíntese diafisária de ossos longos do membro torácico de Tamandua tetradactyla. Autor(es): COSTA, Márcia Cristina da Abstract: The reduction of habitats due to the advance of urban areas and anthropic activities has a direct impact on the reduction of wild fauna. Increasing the casuistry of traumas due to being run over, burns, hunting, dog attacks, among others. Thus, there is an increase in clinical-surgical consultations and interventions in the veterinary routine. Seven fixed specimens were used for macroscopic description of the musculature and identification of surgical approaches for diaphyseal osteosynthesis of long bones of the thoracic limb of Tamandua tetradactyla. The suggested accesses for diaphyseal osteosynthesis were: mediocranial approach to the humerus, to the radius the approach was craniolateral, in turn, to the ulna a laterocaudal intervention is indicated. Such information aims to contribute with anatomical knowledge, fundamental for the assertive choice in the clinical-surgical management of T. tetradactyla.

Identificação molecular de Bartonella spp. no tecido hepático de morcegos da Amazônia brasileira.

2025-11-25T17:29:01Z

Título: Identificação molecular de Bartonella spp. no tecido hepático de morcegos da Amazônia brasileira. Autor(es): PARDAUIL, Suellen de Oliveira Abstract: Bartonellosis is a vector-borne global zoonosis caused by Bartonella, a genus of intracellular Gram-negative bacteria. It is a re-emerging and neglected infectious disease. It infects mammals and is transmitted by arthropod vectors, causing nonspecific clinical manifestations. Several Bartonella genotypes have been identified in several wild mammal species, and bats are a significant reservoir for numerous pathogens, including Bartonella spp. They are a group of mammals with a crucial role in the origin and spread of Bartonella bacteria across geographic regions and among other mammalian groups. In bats, several arthropod taxa are found that feed on blood, which can help in the dispersion of this bacterium. Recognizing the limitations associated with the detection of Bartonella species mainly by means of a single gene and employing only one detection method, conventional PCR was implemented and two gene regions (gltA and ITS) with high specificity and sensitivity were used, allowing a reliable analysis of Bartonella diversity. The present study investigated the occurrence and molecular identity in liver samples (n = 124) of bats of 32 different species from the Amazon region. Amplification of the ITS region revealed the presence of Bartonella spp. DNA in 6 bats 4.33% (6/124) the species were Artibeus jamaicensis, Carollia brevicauda, Glossophaga soricina alopecia, Mesophylla macconnelli, Molossus molossus, Sturnira lilium and Uroderma bilobaton. Partial analysis of the gltA gene evaluated the genetic variants of Bartonella in 1 of the ITS-positive samples of the species Sturnira lilium. A prevalence rate of 5.64% (7/124) positive for Bartonella sp. was detected and showed homology with two species from Brazil and three from Guatemala. This multifaceted approach aimed to provide a more comprehensive understanding of the prevalence of Bartonella species among small mammals. Thus, a correlation was found between synanthropic behavior and the prevalence of Bartonella spp., suggesting that there is an increased risk of spillover from bats to humans in floodplain environments.

Avaliação das características reprodutivas e produtivas de ovinos da raça santa Inês, criados na mesorregião do nordeste paraense.

2025-07-11T13:45:20Z

Título: Avaliação das características reprodutivas e produtivas de ovinos da raça santa Inês, criados na mesorregião do nordeste paraense. Autor(es): SOARES, Felipe Nogueira Abstract: Santa Ines sheep may suffer physiological changes due to climate change, influencing directly in its production capacity, In order to evaluate the reproductive performance and productivity of sheep and their relation to variations in the tropical Amazon, 148 matrices were used Santa Inés crossbred pluriparous, aged 24 to 36 months with an average weight of 40,0 ± 4,93 kg and body condition score 2,5 to 3,0, which were randomly divided into four distinct groups G-1, G - 2, G -3 and G-4 and mated by natural service sires with four Santa Ines in the respective periods, January/February (G-1) April / May (G-2), July / August (G-3) and October / November (G- 4). Weights before and after the breeding season there was no difference p > 0.05 differing p <0.05 only in weight before and after birth to 40,75 ± 2,5 to 44,58 ± 6,2kg respectively. Coverage rates and cyclicity at 15 days remained constant over the four periods with an average of 100%, difference p < 0.05 at 30 and 45 days only Cyclicality of G-4 and G-5. The fertility rate of 86,6% to 91,4% In G-1 G-4 showed only a linear growth. Similar behavior was also observed for calving rate of 70% and 87,2% respectively. The mean litter size was lower to 1,0 G - 3 and at most 1,4 G - 1. For the lowest multiple gestation was 0.0% in G - 3 and 4,6% higher in G - 2. Reproductive failure showed the lowest percentage of 2,1% in Group G - 4 and the highest value of 13.3% for G - 1 while mortality was 0,0% of matrices in G-2 and 3 6,7% in G - 1. In lambs there was a higher proportion of females and 60% growth from first to fourth season on weaning rate, without significant difference (p>0.05). As for weights of lambs by sex, we observed higher weight of males over females p < 0.05 birth (3, 2 ± 0, 49 kg and 3,0 ± 0,33 kg) at 15 days (5,1 and 4.8 ± 1,01kg + 0,97kg ) and 30 days (8,1± 2,32 kg and 7,3 ± 1.94kg ) respectively. However, when analyzed between seasons, adding the weight of male and female means were different between seasons (p <0.05) with the exception of birth weight. The productivity indicators show growth in reproductive efficiency from 0,66 to 0,74, between breeding seasons with an average cycle of 0,73. In respect ky lambs/sheep parity kg from 0,24 to 0,33 kg and the ratio of lambs weaned/ewes mated kg from 0,18 to 0,29.