Biblioteca Digital de Teses e Dissertações. https://repositorio.ufra.edu.br/jspui/handle/123456789/16 2026-09-28T20:34:48Z 2026-09-28T20:34:48Z RODRIGUES, Rogério Antônio Ribeiro banca 2 MUNIZ http://lattes.cnpq.br/4993290160435067, José Augusto Pereira Carneiro banca 3 BERTOLO http://lattes.cnpq.br/8351194788389574, Paulo Henrique Leal banca 4 PINHEIRO http://lattes.cnpq.br/0747820203950636, Raul Henrique da Silva https://orcid.org/0000-0003-3221-5017 https://repositorio.ufra.edu.br/jspui/handle/riufra/2913 2026-09-25T17:11:01Z 2026-07-01T00:00:00Z

Título: Diversidade parasitária do intestino delgado de Saimiri sciureus de vida livre na região urbana do município de Belém, Pará Autor(es): RODRIGUES, Rogério Antônio Ribeiro; banca 2 MUNIZ http://lattes.cnpq.br/4993290160435067, José Augusto Pereira Carneiro; banca 3 BERTOLO http://lattes.cnpq.br/8351194788389574, Paulo Henrique Leal; banca 4 PINHEIRO http://lattes.cnpq.br/0747820203950636, Raul Henrique da Silva https://orcid.org/0000-0003-3221-5017 Abstract: There are approximately 133 species of non-human primates in Brazilian territory, of which 80 are found in the Amazon territory. Various species of neotropical primates can be found in the Rodrigues Alves Botanical Garden in Amazônia, as this place is an important representativo of a conservation and preservation environment. These primate species are potential hosts for helminths from various phyla, such as Nematoda, Platyhelminthes and Acanthocephala. The aim of this study is to investigate which helminths occur in Saimiri sciureus from the Rodrigues Alves Botanical Garden in Amazônia, and to describe the histological changes that occur in the intestino of S. sciureus due to the parasitic interaction of these helminths with the intestino. The specimens of S. sciureus were donated by the Rodrigues Alves Botanical Garden of Amazônia, to the Animal Pathology Laboratory (LABOPAT) of the Federal Rural University of Amazônia, after being found dead. These specimens were submitted to necroscopic examination to determine their cause of death. In the small intestino, the presence of helminths from the Nematoda, Platyhelminthes and Acanthocephala phyla were detected. The helminths were fixed in 10% buffered formalin. The parasitized intestino samples were sent to the Histology and Embryology Laboratory (LHEA) at the Federal Rural University of Amazônia to identify the helminths and describe the histological changes using light microscopy and scanning electron microscopy. The nematodes found parasitizing the small intestino of S. sciureus exhibited characteristics of the Family Oxyuridae and the Genus Trypanoxyuris. The platyhelminth helminths belonged to the Cestoda Class, while the acanthocephalan helminths were diagnosed as belonging to the Prosthenorchis Genus. These helminths were responsible for inducing serious morphological changes in the intestinos of the hosts. From the light microscopy analysis of the intestinos parasitized by Prosthenorchis, it was possible to identify the helminth anchored in the mucosal layer, passing through the underlying layers until it reached the muscular layer. Scanning electron microscopy made it possible to analyze the externai morphological characteristics of the helminth and the details of the tissue lesions caused by them. The helminths from the Nematoda and Platyhelminthes phyla will need to be subjected to more in-depth analysis to diagnose the helminth species. The Acanthocephala showed characteristics of the Prosthenorchis elegans species. This helminth is responsible for severo morphological changes and for impairing the vital function of the parasitized organ in S. sciureus.

2026-07-01T00:00:00Z SOUZA, Joanne Moraes de Melo https://repositorio.ufra.edu.br/jspui/handle/riufra/2912 2026-09-24T20:48:41Z 2026-05-29T00:00:00Z

Título: Avaliação da atividade biológica e do perfil metabólico de extratos de calos, células em suspensão e raízes de Boerhavia paniculata por meio da técnica de lipidômica Autor(es): SOUZA, Joanne Moraes de Melo Abstract: This study aimed to investigate the anti-inflammatory and antimicrobial activities of Boerhavia paniculata and compare the chemical profile of extracts of the roots of wild plants with the extracts of in vitro cultures. Roots were collected and the concentrated metabolites were extracted with the solvents hexane, ethyl acetate and hydroalcoholic solution (8:2; v/v). The anti-inflammatory activity of extracts was evaluated by rat paw edema induced by carrageenan and antimicrobial activity was investigated by the disk diffusion in agar method. An induction protocol for friable callus was developed from seeds without tegument under different treatments in medium with half strength MS (½ MS), different concentrations of glucose (0.0; 1.5; 3.0 % w/v) and 2,4-dichlorophenoxyacetic acid (0.0; 2.265; 4.53 µM; w/v) supplemented with coconut water (10%; v/v). Friable calli were used to establish a cell suspension protocol in ½ MS medium supplemented with glucose (1.5%; w/v) and 2,4-D (2.265 µM; w/v), and cell cultures were elicited with Candida albicans and Aspergillus flavus (2%; w/v). The extraction of metabolites from calli and cell suspension was performed in the same manner as for the roots. The chemical profile of hexane extracts from wild plant roots, calli, and elicited and non-elicited cell suspensions was analyzed by lipidomics using GC/MS. None of the root extracts showed antimicrobial activity, but the hexane root extract exhibited expressive anti-inflammatory activity. The chemical profile of the roots showed phytosterols (β-sitosterol and stigmasterol) and fatty acids as major compounds. The best result for friable callus induction was obtained in ½ MS medium containing 2.265 µM of 2,4-D, glucose (1.5%) and coconut water (10%). The establishment of cell cultures was effective. The chemical profile of in vitro cultures was similar to the chemical profile found in the roots of the wild plant. This work contributes to increasing the chemical-pharmacological knowledge and developing in vitro cultivation studies in the species Boerhavia paniculata.

2026-05-29T00:00:00Z CANELAS, Vitória https://repositorio.ufra.edu.br/jspui/handle/riufra/2911 2026-09-25T11:57:39Z 2026-01-01T00:00:00Z

Título: Características hematológicas de seis espécies de quelônios criados em cativeiro no município de Belém-Pa Autor(es): CANELAS, Vitória Abstract: Hematology is the science dedicated to the study of blood, its origin, and its cellular components, allowing for the evaluation of the physiological, immunological, and metabolic state of the organism, as well as assisting in the diagnosis, prognosis, and monitoring of various diseases. Among hematological tests, the complete blood count (CBC) is the most frequently requested, as it provides an overview of the animal's overall condition. In wild animals, the evaluation of the blood smear is considered the most important step in performing this test, since it is at this stage that abnormalities in red blood cells, morphological changes in white blood cells, and the presence of cellular inclusions and hemoparasites can be visualized. Considering the scarcity of specific studies and the great morphological variability of blood cells in wild species, microscopic analysis of the blood smear becomes fundamental for the correct interpretation of hematological results. Thus, the objective of this research is to contribute to specific studies focused on the hematology of Testudines found in the Amazon region through photodocumentation of blood smears, complete blood count, and blood gas analysis, in order to provide greater ease in the analysis, identification of cells, and interpretation of the examinations of these animals. The research was carried out by obtaining blood samples from 6 species of Testudines: Chelonoidis carbonaria (red-footed tortoise), Chelonoidis denticulata (yellow-footed tortoise), Podocnemis expansa (Amazonian turtle), Rhinoclemmys punctularia (perema turtle), Podocnemis unifilis (yellow-spotted river turtle), and Kinosternon scorpioides (scorpion turtle), obtained from the Museu Paraense Emílio Goeldi. Blood smears were performed on all species, without the use of anticoagulants, stained using the Rapid Panoptic® and adapted May-Grünwald-Giemsa techniques, subsequently analyzed and photomicrographed. Complete blood counts and blood gas analyses were performed on the species Jabuti-Tinga, Jabuti-Piranga, and Amazon River Turtle. Complete blood counts were sent to a partner laboratory, and blood gas analyses were performed locally by venipuncture using the Seamaty Blood Gas & Electrolyte Analyzer - Model VG1. Cell identification demonstrated the efficiency of MayGrunwald-Giemsa staining compared to Panoptic Rapid®, in addition to corroborating and adding relevant information about the morphology of the cellular components of the blood of these animals. The complete blood count and blood gas analysis values found in this study corroborated established literature and added new data about chelonians in the Northern region of Brazil.; Hematology is the science dedicated to the study of blood, its origin, and its cellular components, allowing for the evaluation of the physiological, immunological, and metabolic state of the organism, as well as assisting in the diagnosis, prognosis, and monitoring of various diseases. Among hematological tests, the complete blood count (CBC) is the most frequently requested, as it provides an overview of the animal's overall condition. In wild animals, the evaluation of the blood smear is considered the most important step in performing this test, since it is at this stage that abnormalities in red blood cells, morphological changes in white blood cells, and the presence of cellular inclusions and hemoparasites can be visualized. Considering the scarcity of specific studies and the great morphological variability of blood cells in wild species, microscopic analysis of the blood smear becomes fundamental for the correct interpretation of hematological results. Thus, the objective of this research is to contribute to specific studies focused on the hematology of Testudines found in the Amazon region through photodocumentation of blood smears, complete blood count, and blood gas analysis, in order to provide greater ease in the analysis, identification of cells, and interpretation of the examinations of these animals. The research was carried out by obtaining blood samples from 6 species of Testudines: Chelonoidis carbonaria (red-footed tortoise), Chelonoidis denticulata (yellow-footed tortoise), Podocnemis expansa (Amazonian turtle), Rhinoclemmys punctularia (perema turtle), Podocnemis unifilis (yellow-spotted river turtle), and Kinosternon scorpioides (scorpion turtle), obtained from the Museu Paraense Emílio Goeldi. Blood smears were performed on all species, without the use of anticoagulants, stained using the Rapid Panoptic® and adapted May-Grünwald-Giemsa techniques, subsequently analyzed and photomicrographed. Complete blood counts and blood gas analyses were performed on the species Jabuti-Tinga, Jabuti-Piranga, and Amazon River Turtle. Complete blood counts were sent to a partner laboratory, and blood gas analyses were performed locally by venipuncture using the Seamaty Blood Gas & Electrolyte Analyzer - Model VG1. Cell identification demonstrated the efficiency of MayGrunwald-Giemsa staining compared to Panoptic Rapid®, in addition to corroborating and adding relevant information about the morphology of the cellular components of the blood of these animals. The complete blood count and blood gas analysis values found in this study corroborated established literature and added new data about chelonians in the Northern region of Brazil.

2026-01-01T00:00:00Z PACHECO, Acácio Heitor Barros https://repositorio.ufra.edu.br/jspui/handle/riufra/2910 2026-09-24T19:13:21Z 2026-07-15T00:00:00Z

Título: Ocorrência de mixosporídeos em (Mylossoma duriventre) e possíveis implicações fisiológicas na região de Santarém-PA Autor(es): PACHECO, Acácio Heitor Barros Abstract: Mylossoma duriventre (pacu manteiga) is a species widely distributed in the Amazon Basin, of food and ornamental importance in the Northern region. This study aimed to characterize morphologically, histopathologically, and molecularly the myxosporidia that infect M. duriventre from the Santarém-PA region, evaluating their tissue distribution and possible physiopathological implications. One hundred and twenty specimens acquired at the Santarém-PA Fish Market between December 2024 and October 2025 were used, subjected to necropsy, histopathological, molecular, and phylogenetic analyses, as well as biometrics. The laboratory procedures were performed at the Laboratory of Morpho-molecular Integration and Technologies (LIMT) of the Federal Rural University of the Amazon (UFRA, Belém campus). Parasitized tissues were processed for histology, electron microscopy, and molecular biology. Based on spore morphology, representatives of the genera Sphaeromyxa, Myxobolus, Henneguya, and Ortholinea were identified, distributed in different organs, including the kidney, gills, and urinary bladder. Histological analyses revealed tissue alterations compatible with myxozoan infection, especially in the gills, suggesting potential local functional impairment. However, no parasitic structures or histopathological alterations were observed in the gonads of the analyzed individuals. Therefore, it is recommended that future studies include experimental designs and physiological biomarkers capable of directly and quantitatively evaluating possible interactions between parasitism and the reproductive performance of M. duriventre.

2026-07-15T00:00:00Z