Germinação in vitro de camu-camuzeiro (Myrciaria dubia (H.B.K.) Mc Vaugh).

Abstract

The success of tissue culture techniques is sometimes dependent on the previous establishment of the culture to be studied in vitro. The main problem for the production of aseptic plantlets is the high contamination of index explants obtained directly from the field. This research had the objective to evaluate the germination percentage of explants of camu-camu from two in vitro germination experiments in order to obtain aseptic plantlets. In the first experiment, a complete randomized experimental design in a 5x5 factorial scheme, with five concentrations of gibberillic acid (GA,) applied to five kinds of explants from ripe seeds of camu-camu, was used. In the second experiment, a complete randomized experimental design in 2x3x4 factorial scheme, with presence or absence of activated coal at 3g.L1, seeds in three ripening phases and four concentrations of GA, (0,0; 1,0; 2,0 e 4,0 mg.L1, was used. The explants were inoculated in MS medium with pH of 5,8 containing 20g.L1 of sucrose and 2g.L1 of phytagel. The incubation was in a room with photoperiod of 16 H/light/day and temperature of 25º + 2ºC. The number of germinated explants was low independent of the ripening phase, the presence of GA, and of activated coal in the culture medium.