Terapia celular em cães com displasia coxofemoral.

Abstract

Coxofemoral dysplasia is a disease characterized by the abnormal development of the joint of the coxal, very common in medium-sized dogs, resulting in joint degeneration. The treatment of the disease includes a variety of therapeutic options, however, none is considered ideal for the definitive improvement of the patient. Due to the potential for differentiation, self-renewal capacity and high plasticity, mesenchymal stem cells (MSCs) have been considered as a treatment option in the repair of cartilage lesions. The aim of this study was to evaluate the clinical improvement of dogs diagnosed with coxofemoral dysplasia and submitted to intraarticular infusion of autologous mesenchymal stem cells derived from adipose tissue. Four adult dogs were used, diagnosed by bilateral radiography. After harvesting adipose tissue from each patient, mesenchymal stem cells were isolated by enzymatic digestion, cultured and expanded, viability tested, morphology, differentiation, immunophenotyping and chromosomal stability were analyzed. The CTMs were infused into the intra-articular space of the hip joint, in the amount of 1x106 cells, with an interval of approximately 18 days between the infusions, in a total of two infusions. All animals submitted to intra-articular stem cell infusions showed improvement when the clinical characteristics of the disease, however, no changes were observed in the radiographic and ultrasonographic patterns of the animals evaluated.