Uso do plasma rico em fibrina associado ao cloridrato de tramadol na lesão medular aguda em ratos.

Abstract

Spinal cord injury is considered one of the main causes of immobility in dogs and cats, occurring mainly due to accidents, and appears as one of the most serious and debilitating pathological conditions of the animal. The search for treatments that minimize the neurological damages that occur and stimulate the recovery of the affected patient are a constant. The results obtained with the used drugs have been divergent, favoring the research with other therapies such as the fibrin-rich plasma, which has been applied in the repair of several tissues, allowing a better cicatrization. Tramadol hydrochloride is an analgesic widely used in musculoskeletal and neurological lesions, conferring a multimodal analgesia, besides being more secure in relation to other opioids. Tramadol has recently been described as an antidematogenic action, suggesting the possibility of it’s beneficial action on the spinal cord injury. The objective of this project was to analyze the therapeutic potential using fibrin-rich plasma and its association with tramadol hydrochloride in the acute phase of bone marrow injury in adult rats. Twenty - three male Wistar rats were divided into 4 groups: GCo - control, GMF - fibrin matrix, GMFTr - tramadol - associated fibrin matrix, GTr - tramadol. The animals were submitted to hemisection of the spinal cord at the T8 level and treated according to the proposed group, being evaluated 24 hours, 3, 7 and 14 days after the injury, and applied the Grimace and BBB scales for pain and functional evaluation respectively. The results showed functional improvement when the 24-hour and 14-day postoperative days were compared. However, no difference was found between the groups regarding motor function. There was an intense negative correlation between the results of the BBB scale and the Grimace Scale in the GCo and a large negative correlation in the tramadol group. In the histopathological study the GTr showed better tissue preservation, followed by the GMFTr group in comparison to the others. There was no improvement in the functional evaluation of the animals with the use of the fibrin matrix or its association with tramadol, however the use of the analgesic considerably reduced the pain levels of the animals. In the histopathological study it was identified that GTr and GMFTr presented better tissue preservation.