Identification of Chromobacterium violaceum genes with potential biotechnological application in environmental detoxification

Abstract

Chromobacterium violaceum is a Gram-negative bac terium found in a wide variety of tropical and subtropical ecosystems. The complete genome sequence of C. violaceum ATCC 12472 is now available, and it has considerable biotechnological potential for various applications, such as environmental detoxification, as well as medical and agricultural use. We examined the biotechnological potential of C. violaceum for environmental detoxification. Three operons, comprising the ars operon, involved in arsenic resistance, the cyn operon, involved in cyanate detoxification, and the hcn operon, encoding a cyanase, re sponsible for biogenic production of cyanide, as well as an open reading frame, encoding an acid dehalogenase, were analyzed in detail. Prob able catalytic mechanisms for the enzymes were determined, based on amino acid sequence comparisons and on published structural informa tion for these types of proteins.