Estudo morfológico, molecular e filogenético de microparasitos em Geneiosus ucayalensis Castelnau, 1855, capturados no reservatório da UHE Coaracy Nunes, Estado do Amapá, Amazônia Oriental.

Abstract

The Amazon coastal ecosystem is considered biologically important due to the abundance of fish in the estuary and the rich and diverse fish fauna. Native fish are susceptible to being infected by different types of icroparasites because their habitats are conducive to their reproduction and development. Among which we can mention those belonging to the phylum Cnidaria, class Myxozoa. Myxosporidia are found in most organs of their hosts' bodies, the most common being the swim bladder, urinary bladder, gallbladder, liver, gall kidney and gills. In this sense, the present project sought to contribute to the identification of the types of eukaryotic micropastes of the Myxozoa class, found in the specimen Ageneiosus ucayalensis from the Araguari river, UHE Coaracy Nunes, municipality of Ferreira Gomes, in the state of Amapá. 35 specimens of Ageneiosus ucayalensis were captured by the Amazon Aquatic Organism Health (SOAA) research team and assisted by local fishermen using a 30 mm trawl net, cast nets and fishing reels. The individuals were packaged and transported alive to the Morphophysiology and Animal Health Laboratory (LABMORSA) of the University of the State of Amapá (UEAP), anesthetized MS 222 SIGMA and necropsied for microscopic analyzes (CEUA no 8323110522). Small fragments of the hosts' infected organs were removed with the help of tweezers, fixed in Davidsom for 24 hours and 80% alcohol to be processed for histology and Molecular Biology (BM) respectively. The prevalence of infection in the urinary bladder of Ageneiosus ucayalensis specimens was 28/35 (80%), they were contaminated with plasmodia and spores of the genus Hoferellus, Berg 1898 belonging to the phylum Cnidaria (Class Myxozoa). Morphological, morphometric and phylogenetic analyzes were carried out using 18s rDNA and it was found that they did not correspond to any species already described in the literature, thus Hoferellus matosi n. sp. It is the third taxon found in Brazil. The microparasite found in this study highlights the importance of new large studies of ichthyoparasites in the Araguari River in the Municipality of Ferrreira Gomes – Amapá, Amazon region.