O Repositório Institucional da Universidade Federal Rural da Amazônia (RIUFRA) é um dispositivo de armazenamento e disseminação das obras intelectuais da UFRA, produzidas no âmbito das atividades de pesquisa, ensino e extensão da instituição. É composto de documentos em formato digital, provenientes das atividades desenvolvidas pelo corpo docente, discente e técnico-administrativo da UFRA e por obras elaboradas a partir de convênio ou colaboração entre a instituição e outros órgãos publicados em autoria e/ou coautoria.
Please use this identifier to cite or link to this item:
http://repositorio.ufra.edu.br/jspui/handle/123456789/2296| Title: | New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters |
| Authors: | AZEVEDO, Juliana Simão Nina de OLIVEIRA, Carlos Sousa Oliveira, Carlos Sousa |
| Keywords: | Estuary IncP-1 plasmid Broad-host-range-plasmid trfA gene Surface sea microlayer Ria de Aveiro |
| Issue Date: | 2012 |
| Publisher: | Elsevier |
| Citation: | AZEVEDO, Juliana Simão Nina de et al. New molecular variants of epsilon and beta IncP-1 plasmids are present in estuarine waters. Plasmid, v. 67, p. 252–258, 2012. Disponível em: http://repositorio.ufra.edu.br/jspui/handle/123456789/2296. Acesso em: |
| Abstract: | In this work the presence of broad-host-plasmids in an estuary in Portugal has been inves tigated. Pseudomonas putida KT2442 was used as model recipient bacteria in biparental matings with tetracycline and mercury to select for resistance phenotypes. As a result, 7 transconjugants were shown to carry broad-host-plasmids from the IncP-1 group, as seen by PCR amplification of the trfA gene. Sequence analysis confirmed the isolation of 4 plas mids from b-1 subgroup and 3 assigned to the recently described e subgroup. To our knowledge this is the first report concerning the detection and isolation of IncP-1b and e plasmids in estuarine waters. Moreover it is shown that, even though the retrieved plas mids are phylogenetically close to previously characterized plasmids, such as pB10 and pKJK5, respectively, they constitute new molecular variants. |
| URI: | http://repositorio.ufra.edu.br/jspui/handle/123456789/2296 |
| Appears in Collections: | Capanema - Artigos Científicos |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| New molecular variants of epsilon and beta IncP-1 plasmids are present.pdf | 428,5 kB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License
