Montagem, predição gênica e anotação do genoma da Cynoscion acoupa (Lacepède, 1801).

Abstract

Aquaculture in Brazil had positive results in 2023, where national production reached 887,03 tons. The Cynoscion acoupa is a fish popularly known as “pescada-amarela”, found mainly on the north coast and has a great economic value mainly for gastronomic purposes. More recently, a material called "isinglass" was observed, found in the swim bladder of this fish, which has been used as raw material in several areas of the market, such as the pharmaceutical, civil, food, among others. The purpose of this work is to perform the genome assembling, gene prediction and carry out functional annotation of the C. acoupa organism. DNA samples were extracted from 5 specimens in the regions of Salinópolis-PA, Bragança-PA and on the coast of Amapá. The best sample was chosen to continue the study. Through these samples, the complete genome of the captured individuals was sequenced, with two paired libraries paired-end short insert (2 x 250 bp) DNAseq built with the Illumina DNA prep Kit, using the NovaSeq SP 6000 Illumina sequencing platform, producing raw data. Subsequently, FASTQC were used to verify the quality of the sequencing. From the generated sequences the de novo genome assembly will be made using the ABYSS assembler. BOWTIE-2 will be used to align the contigs and scaffolds with the genome of a phylogenetically close species, GeneMark will be used for gene prediction and BUSCO for genome annotation, so that it can finally be deposited at the NCBI. Through the results, it is expected to bring new information about genome sequencing and its quality, together with the publication of these data for future studies.