Determinação quantitativa da microbiota bacteriana prepucial e vaginal em macacos-da-noite(Aotus azarai infulatus) criados em cativeiro.

Abstract

The pathogenic potential about different micro-organisms, the reports os microbial resistance from the genital treat of not human primates and lack of information are factors that justify the reason for this study. The purpose for this study was to characterize the vaginal and prepuce aerobic microbiota from Nighty Monkeys (Aotus azarai infulatus) raised in captivity, valuating the frequency between sex and social organization (mated or not), besides to evaluate the antibacterial sensibility. The samples were collected from 30 adult and healthy individuais,that are raised on the National Center of Primates, in the municipality of Ananindeua on the State of Pará, Brazil using swabs to do it so. They were sown and incubated in aerobics with blood agar, MacConkey agar and Chapman agar, to bacterial identification and the sensibility tests were done by the automatic system VITEK® 2 Compact. The results were checked using the estimators of richness and diversity of species, on the comparisons were used the qui-squared (X2) with (p < 0,05). Between the isolated gram positive strains the Staphylococcus intermedius had the major frequency among females and males (48,05% e 30,30%, respectively). Between the gram negative strains the Proteus mirabilis had major frequency on females (27,10%) and males (31,34%). The same species were the most found in not mated animais (S. intermedius: 41,33% e P. mirabilis: 29,17%) and mated (S. intermedius: 38,23% e P. mirabilis: 27,85%). To the gram positive strains the standard antimicrobial sensibility in vitro were the same for both genders, showing 100% of resistance to benzilpenicilina and 100% de of sensigility against gentamicina, ciprofloxacina and norfloxacina. To the gram negative strains, he females showed 100% of sensibility against levofloxacina and the males against five antibiotics (cefepima, meropenem, amicacina, ciprofloxacina and levofloxacina), both genders were less sensitive to nitrofurantoína (females: 33,70% and males: 25,40%). The data analysis showed that are similiarity between the aerobic bacterial composition of the prepuce and vaginal mucosa from A a. infulatus, and the numbers of isolations and bacterial species don't vary related to gender and social organization and the standard antimicrobial sensibility were the same among genders to gram positive and different for gram negative strains.