Pesquisa de rotavírus em aves silvestres da região amazônica mantidas em cativeiro no estado do Pará.

Abstract

The present study aims to identify the presence of rotavirus in wild birds kept in captivity in Pará State (PA), as well as investigate rotavirus circulating groups in fecal specimens of these birds, to characterize the rotavirus electropherotypes found, and specifically investigate the presence of groups A and D rotavirus in fecal specimens Fecal samples were collected at the "Fazenda Paricuiã" (Terra Alta-PA), "Jardim Zoobotânico da Amazônia Bosque Rodrigues Alves"; "Parque Ecológico Mangai das Garças" and "Museu Paraense Emílio Goeldi (MPEG)" at Belém/Pará State/Brazil, in the period between March 2011 and February 2012. We collected fecal samples from 83 birds belonging to the orders: Psittaciformes (Psittacidae family), Ciconiformes (Ardeidae and Threskiornithidae family) and Falconiformes (Accipitridae family). Fecal suspensions were prepared from the samples collected, with subsequent extraction of viral dsRNA, which was subjected to polyacrylamide gel electrophoresis (PAGE). The chain reaction mediated polymerase and reverse transcription (RT-PCR) was performed with primers specific for amplification of the NSP4 gene of A rotavirus and VP6 of D rotavirus. Ali samples were negative by both EGPA and by RT-PCR, requiring, however, further studies aimed at detection of rotavirus in wild birds and contribute to knowledge about the role of these species in the epidemiology of the disease.