Isolamento e caracterização de células-tronco mesenquimais derivadas do tecido adiposo de Chlorocebus aethiops.

Abstract

The mesenchymal stem cells (MSCs) are rare and undifferentiated cells, possess high potential for self-renewal, proliferation and differentiation. This plasticity could allow the use of such cells in regenerative medicine. The objective was to isolate, characterize and differentiate Chlorocebus aethiops adipose tissue-derived MSCs. MSCs were isolated by enzymatic digestion using type I collagenase and in vitro cultured until passage 4 in Dulbecco's Modified Eagle's Médium supplemented with 10% fetal bovine serum. It was performed cell growth curve and, subsequently evaluation for senescence, through the activity of the enzime P-galactosidase, and chromosomal integrity. Cells were characterized by presence of surface receptors for MSCs and also to the absence of specific markers of hematopoietic cells. The differentiation into adipocytes, chondrocytes and osteocytes was induced in vitro and was assessed by specific staining. The results demonstrated that C. aethiops adipose-derived MSCs have the ability to expand in culture, proliferating fibroblastoid-shaped along the passages. It was observed percentage of senescent cells (12.5%), but without chromosomal alteration. The cells were positive for specific markers of MSCs and demonstrated mesodermal lineages differentiation potential, proving the multipotency of C. aethiops adipose tissue- derived MSCs.