Densidade e diversidade bioquímica de rizobacérias associadas A Bellucia grossuairioides (L). em áreas nativas e reflorestadas na base de Urucu, Coari-AM.

Abstract

To minimize the impacts caused by the process of degradation caused by oil exploration. use native species are hardy, tolerant of dry periods and low soil fertility, and capable of producing large quantities of organic matter in reforestation. The species of tapir Guava (Bellucia grossularioides). It has great importance in the recovery process. Aiming to study the biochemicai diversity of rhizobacteria associated with Bellucia grossularioides in native and reforested areas, on the basis of Urucu Coari, AM, was held this job. Rhizosphere soils were collected in layers 0-20 cm deep in the rainy and dry in 14 areas (seven forested areas, four areas of fields and wooded glades reforested areas 3). From these soils were 118 bacterial isolates, which underwent the following assessments: CFU (colony forming unit), phenotypic characterization, biochemical characterization associated with the promotion of growth: production of indole acetic acid (IÃA), siderophores, and cytokinins gibberellins and enzymatic activity: the fixation of nitrogen (BEN), cellulolytic activity, proteolytic and amylase, phosphate solubilization (SF) and acid phosphatase (FAQ). Also studied were the Microbiological parameters of soil respiration (BR), microbial biomass carbon (MBC) and metabolic quotient gCO2, the three areas concerned. The variables of population level UFC, microbiological attributes and the Shannon index showed that areas of native forest has the highest density and diversity of rhizobacteria associated with B. grossularioides in both seasons. The microorganisms of the carbon cycle are more present in areas of recovery during the dry season. In the rainy season are more present in the forested area. Nitrogen fixation was higher in the two sampling periods, areas of clearing. The evaluation of acid phosphatase activity was higher in the forested area. However, phosphate solubilizing rhizobacteria occurred in greater density in areas of deposit and clearing during the dry season and in areas of forest and field in the rainy season. Biochemical tests associated with the promotion of growth showed that in general areas of forest and meadow, a higher percentage of rhizobacteria with these characteristics in both collection periods. With the phenotypic characterization, 43 groups were determined phenotypically identical among isolates within each group. The functional groups do not have the rhizobacteria isolates belonging to the same area, but distributed in all areas studied.